[Source: Cell Host & Microbe, full page: (LINK). Abstract, edited.]
Dietary Manganese Promotes Staphylococcal Infection of the Heart
Lillian J. Juttukonda, Evelien T.M. Berends, Joseph P. Zackular, Jessica L. Moore, Matthew T. Stier, Yaofang Zhang, Jonathan E. Schmitz, William N. Beavers, Christiaan D. Wijers, Benjamin A. Gilston, Thomas E. Kehl-Fie, James Atkinson, Mary K. Washington, R. Stokes Peebles, Walter J. Chazin, Victor J. Torres, Richard M. Caprioli, Eric P. Skaar9
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Publication stage: In Press Corrected Proof / DOI: http://dx.doi.org/10.1016/j.chom.2017.08.009
Article Info:
Publication History: Published: September 21, 2017 – Accepted: August 17, 2017 – Received in revised form: June 29, 2017 – Received:March 15, 2017
Highlights
- High levels of dietary manganese increase lethality and heart colonization by S. aureus
- Calprotectin does not alter manganese availability in the heart
- Mice fed a high-manganese diet have increased bioavailable manganese in the heart
- S. aureus utilizes dietary manganese to protect against ROS and neutrophil killing
Summary
Diet, and specifically dietary metals, can modify the risk of infection. However, the mechanisms by which manganese (Mn), a common dietary supplement, alters infection remain unexplored. We report that dietary Mn levels dictate the outcome of systemic infections caused by Staphylococcus aureus, a leading cause of bacterial endocarditis. Mice fed a high Mn diet display alterations in Mn levels and localization within infected tissues, and S. aureus virulence and infection of the heart are enhanced. Although the canonical mammalian Mn-sequestering protein calprotectin surrounds staphylococcal heart abscesses, calprotectin is not released into the abscess nidus and does not limit Mn in this organ. Consequently, excess Mn is bioavailable to S. aureus in the heart. Bioavailable Mn is utilized by S. aureus to detoxify reactive oxygen species and protect against neutrophil killing, enhancing fitness within the heart. Therefore, a single dietary modification overwhelms vital host antimicrobial strategies, leading to fatal staphylococcal infection.
Keywords: nutritional immunity, diet, manganese, calprotectin, bacterial pathogenesis, Staphylococcus aureus, endocarditis, oxidative stress, neutrophils
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Keywords: Staphylococcus Aureus; Endocarditis; Animal Models.
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