Cooperation of the #Ebola proteins VP40 and GP1,2with BST2 to activate NF-κβ independently of virus-like particle trapping (J Virol., abstract)

[Source: Journal of Virology, full page: (LINK). Abstract, edited.]

Cooperation of the Ebola proteins VP40 and GP1,2with BST2 to activate NF-κβ independently of virus-like particle trapping

Maryan G. Rizk a, Christopher F. Basler b and  John Guatelli a,c#

Author Affiliations: University of California San Diego, La Jolla, CA, USA{a}; Center for Microbial Pathogenesis, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA{b}; Veterans Affairs San Diego Healthcare System, San Diego, CA, USA{c}

 

ABSTRACT

BST2 is a host protein with dual functions in response to viral infections: it traps newly assembled enveloped virions at the plasma membrane of infected cells, and it induces NF-κβ activity, especially in the context of retroviral assembly. In this study, we examined whether Ebola virus proteins affect BST2-mediated induction of NF-κβ. We found that the Ebola matrix protein, VP40, and envelope glycoprotein, GP, each cooperate with BST2 to induce NF-κβ activity, with maximal activity when all three proteins are expressed. Unlike the Human Immunodeficiency Virus type 1 Vpu protein, which antagonizes both virion-entrapment and the activation of NF-κβ by BST2, Ebola GP does not inhibit NF-κβ signaling even while it antagonizes the entrapment of virus-like particles. GP from Reston Ebola virus, a non-pathogenic species in humans, showed a similar phenotype to GP from Zaire Ebola, a highly pathogenic species, in terms of both the activation of NF-κβ and the antagonism of virion-entrapment. Although Ebola VP40 and GP both activate NF-κβ independently of BST2, VP40 is the more potent activator. Activation of NF-κβ by the Ebola proteins either alone or together with BST2 requires the canonical NF-κβ signaling pathway. Mechanistically, the maximal NF-κβ activation by GP, VP40, and BST2 together requires the ectodomain cysteines needed for BST2-dimerization, the putative BST2 tetramerization residue L70, and Y6 of a potential hemi-ITAM motif in BST2’s cytoplasmic domain. BST2 with a GPI anchor signal-deletion, which is not expressed at the plasma membrane and is unable to entrap virions, activated NF-κβ in concert with the Ebola proteins at least as effectively as wild type BST2. Signaling by the GPI-anchor mutant also depended on Y6 of BST2. Overall, our data show that activation of NF-κβ by BST2 is independent of virion-entrapment in the case of Ebola virus. Nonetheless, BST2 may induce or amplify proinflammatory signaling during Ebola virus infection, potentially contributing to the dysregulated cytokine response that is a hallmark of Ebola virus disease.

 

IMPORTANCE

Understanding how the host responds to viral infections informs the development of therapeutics and vaccines. We asked how proinflammatory signaling by the host protein BST2/tetherin, which is mediated by the transcription factor NF-κβ, responds to Ebola proteins. Although the Ebola virus envelope glycoprotein (GP1,2) antagonizes the trapping of newly formed virions at the plasma membrane by BST2, we found that it does not inhibit BST2’s ability to induce NF-κβ activity. This distinguishes GP1,2 from the HIV-1 protein Vpu, the prototype BST2-antagonist, which inhibits both virion-entrapment and the induction of NF-κβ activity. Ebola GP1,2, the Ebola matrix protein VP40, and BST2 are at least additive with respect to the induction of NF-κβ activity. The effects of these proteins converge on an intracellular signaling pathway that depends on a protein-modification termed neddylation. Better mechanistic understanding of these phenomena could provide targets for therapies that modulate the inflammatory response during Ebola virus disease.

 

FOOTNOTES

#Address correspondence to John Guatelli, jguatelli@ucsd.edu

Copyright © 2017 American Society for Microbiology. All Rights Reserved.

Keywords: Ebola.

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gimi69

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.

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