[Source: Eurosurveillance, full page: (LINK). Abstract, edited.]
Eurosurveillance, Volume 22, Issue 32, 10 August 2017 / Surveillance and outbreak report
Reoccurrence of botulinum neurotoxin subtype A3 inducing food-borne botulism, Slovakia, 2015
L Mad’arová 1 , BG Dorner 2 3 , L Schaade 3 , V Donáth 4 , M Avdičová 1 , M Fatkulinová 1 , J Strhársky 1 , I Sedliačiková 1 , C Klement 1 5 , MB Dorner 2 3
Author affiliations: 1. Regional Authority of Public Health Banská Bystrica, Banská Bystrica, Slovakia; 2. Robert Koch Institute, Consultant laboratory for neurotoxin-producing clostridia (botulism, tetanus), Berlin, Germany; 3. Robert Koch Institute, Centre for Biological Threats and Special Pathogens, Berlin, Germany; 4. F. D. Roosevelt Teaching Hospital, Department of Neurology, Slovak Medical University, Banská Bystrica, Slovakia; 5. Slovak Medical University, Faculty of Public Health, Bratislava, Slovakia
Correspondence: Martin Bernhard Dorner (firstname.lastname@example.org)
Citation style for this article: Mad’arová L, Dorner BG, Schaade L, Donáth V, Avdičová M, Fatkulinová M, Strhársky J, Sedliačiková I, Klement C, Dorner MB. Reoccurrence of botulinum neurotoxin subtype A3 inducing food-borne botulism, Slovakia, 2015. Euro Surveill. 2017;22(32):pii=30591. DOI: http://dx.doi.org/10.2807/1560-7917.ES.2017.22.32.30591
Received:10 June 2016; Accepted:25 November 2016
A case of food-borne botulism occurred in Slovakia in 2015. Clostridium botulinum type A was isolated from three nearly empty commercial hummus tubes. The product, which was sold in Slovakia and the Czech Republic, was withdrawn from the market and a warning was issued immediately through the European Commission’s Rapid Alert System for Food and Feed (RASFF). Further investigation revealed the presence of botulinum neurotoxin (BoNT) subtype BoNT/A3, a very rare subtype implicated in only one previous outbreak (Loch Maree in Scotland, 1922). It is the most divergent subtype of BoNT/A with 15.4% difference at the amino acid level compared with the prototype BoNT/A1. This makes it more prone to evading immunological and PCR-based detection. It is recommended that testing laboratories are advised that this subtype has been associated with food-borne botulism for the second time since the first outbreak almost 100 years ago, and to validate their immunological or PCR-based methods against this divergent subtype.
Keywords: Clostridium Botulinum; Botulism; Slovakia.