[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
PLoS One. 2016 Sep 15;11(9):e0162375. doi: 10.1371/journal.pone.0162375.
Novel Reassortant H5N6 Influenza A Virus from the Lao People’s Democratic Republic Is Highly Pathogenic in Chickens.
Butler J1, Stewart CR1, Layton DS1, Phommachanh P2, Harper J1, Payne J1, Evans RM1, Valdeter S1, Walker S1, Harvey G1, Shan S1, Bruce MP1, Rootes CL1, Gough TJ1, Rohringer A1, Peck GR1, Fardy SJ1, Karpala AJ1, Johnson D1, Wang J1, Douangngeun B2, Morrissy C1, Wong FY1, Bean AG1, Bingham J1, Williams DT1.
Author information: 1CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia. 2National Animal Health Laboratory, Vientiane, Lao People’s Democratic Republic.
Avian influenza viruses of H5 subtype can cause highly pathogenic disease in poultry. In March 2014, a new reassortant H5N6 subtype highly pathogenic avian influenza virus emerged in Lao People’s Democratic Republic. We have assessed the pathogenicity, pathobiology and immunological responses associated with this virus in chickens. Infection caused moderate to advanced disease in 6 of 6 chickens within 48 h of mucosal inoculation. High virus titers were observed in blood and tissues (kidney, spleen, liver, duodenum, heart, brain and lung) taken at euthanasia. Viral antigen was detected in endothelium, neurons, myocardium, lymphoid tissues and other cell types. Pro-inflammatory cytokines were elevated compared to non-infected birds. Our study confirmed that this new H5N6 reassortant is highly pathogenic, causing disease in chickens similar to that of Asian H5N1 viruses, and demonstrated the ability of such clade 2.3.4-origin H5 viruses to reassort with non-N1 subtype viruses while maintaining a fit and infectious phenotype. Recent detection of influenza H5N6 poultry infections in Lao PDR, China and Viet Nam, as well as six fatal human infections in China, demonstrate that these emergent highly pathogenic H5N6 viruses may be widely established in several countries and represent an emerging threat to poultry and human populations.
PMID: 27631618 DOI: 10.1371/journal.pone.0162375
[PubMed – as supplied by publisher]
Keywords: Research; Abstracts; Avian Influenza; H5N6; Poultry; Laos; Reassortant Strain.