#Genetic and #antigenic characterization of #H5, #H6 and #H9 #avian #influenza viruses circulating in live #bird #markets with intervention in the center part of #Vietnam (Vet Microbiol., abstract)

[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]

Vet Microbiol. 2016 Aug 30;192:194-203. doi: 10.1016/j.vetmic.2016.07.016. Epub 2016 Jul 25.

Genetic and antigenic characterization of H5, H6 and H9 avian influenza viruses circulating in live bird markets with intervention in the center part of Vietnam.

Chu DH1, Okamatsu M2, Matsuno K3, Hiono T2, Ogasawara K2, Nguyen LT2, Van Nguyen L4, Nguyen TN4, Nguyen TT4, Van Pham D4, Nguyen DH4, Nguyen TD4, To TL4, Van Nguyen H5, Kida H6, Sakoda Y7.

Author information: 1Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan; Department of Animal Health, Ministry of Agriculture and Rural Development, 15/78 Giai Phong, Phuong Mai, Dong Da, Hanoi, Vietnam. 2Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan. 3Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Hokkaido 001-0020, Japan. 4Department of Animal Health, Ministry of Agriculture and Rural Development, 15/78 Giai Phong, Phuong Mai, Dong Da, Hanoi, Vietnam. 5Sub-Department of Animal Health, 62 Nguyen Chi Dieu, Hue city, Vietnam. 6Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Hokkaido 001-0020, Japan; Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan. 7Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Hokkaido 001-0020, Japan. Electronic address: sakoda@vetmed.hokudai.ac.jp.

 

Abstract

A total of 3,045 environmental samples and oropharyngeal and cloacal swabs from apparently healthy poultry have been collected at three live bird markets (LBMs) at which practices were applied to reduce avian influenza (AI) virus transmission (intervention LBMs) and six conventional LBMs (non-intervention LBMs) in Thua Thien Hue province in 2014 to evaluate the efficacy of the intervention LBMs. The 178 AI viruses, including H3 (19 viruses), H4 (2), H5 (8), H6 (30), H9 (114), and H11 (5), were isolated from domestic ducks, muscovy ducks, chickens, and the environment. The prevalence of AI viruses in intervention LBMs (6.1%; 95% CI: 5.0-7.5) was similar to that in non-intervention LBMs (5.6%; 95% CI: 4.5-6.8; χ(2)=0.532; df=1; P=0.53) in the study area. Eight H5N6 highly pathogenic avian influenza (HPAI) viruses were isolated from apparently healthy ducks, muscovy ducks, and an environmental sample in an intervention LBM. The hemagglutinin genes of the H5N6 HPAI viruses belonged to the genetic clade 2.3.4.4, and the antigenicity of the H5N6 HPAI viruses differed from the H5N1 HPAI viruses previously circulating in Vietnam. Phylogenetic and antigenic analyses of the H6 and H9 viruses isolated in both types of LBMs revealed that they were closely related to the viruses isolated from domestic birds in China, Group II of H6 viruses and Y280 lineage of H9 viruses. These results indicate that the interventions currently applied in LBMs are insufficient to control AI. A risk analysis should be conducted to identify the key factors contributing to AI virus prevalence in intervention LBMs.

Copyright © 2016 Elsevier B.V. All rights reserved.

KEYWORDS: Antigenic analysis; Avian influenza; Live bird market; Phylogenetic analysis; Surveillance

PMID: 27527783 DOI: 10.1016/j.vetmic.2016.07.016

[PubMed – in process]

Keywords: Research; Abstracts; Avian Influenza; H5N6; H5N1; H5; H6; H9; Reassortant Strains; Poultry; Vietnam.

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Giuseppe Michieli

I am an Italian blogger, active since 2005 with main focus on emerging infectious diseases such as avian influenza, SARS, antibiotics resistance, and many other global Health issues. Other fields of interest are: climate change, global warming, geological and biological sciences. My activity consists mainly in collection and analysis of news, public services updates, confronting sources and making decision about what are the 'signals' of an impending crisis (an outbreak, for example). When a signal is detected, I follow traces during the entire course of an event. I started in 2005 my blog ''A TIME'S MEMORY'', now with more than 40,000 posts and 3 millions of web interactions. Subsequently I added an Italian Language blog, then discontinued because of very low traffic and interest. I contributed for seven years to a public forum (FluTrackers.com) in the midst of the Ebola epidemic in West Africa in 2014, I left the site to continue alone my data tracking job.